SrasV1, Main, Exploration, bibRecord, 004874

Design of recombinant protein-based SARS-CoV entry inhibitors targeting the heptad-repeat regions of the spike protein S2 domain.

Identifieur interne : 004874 ( Main/Exploration ); précédent : 004873; suivant : 004875

Design of recombinant protein-based SARS-CoV entry inhibitors targeting the heptad-repeat regions of the spike protein S2 domain.

Auteurs : Ling Ni [République populaire de Chine] ; Jieqing Zhu ; Junjie Zhang ; Meng Yan ; George F. Gao ; Po Tien

Source :

Biochemical and biophysical research communications [ 0006-291X ] ; 2005.

RBID : pubmed:15781229

Descripteurs français

KwdFr :
- Dichroïsme circulaire, Fusion membranaire (), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (antagonistes et inhibiteurs), Humains, Lignée cellulaire, Protéines de l'enveloppe virale (antagonistes et inhibiteurs), Protéines recombinantes (pharmacologie), Virus du SRAS (physiologie), Électrophorèse sur gel de polyacrylamide.
MESH :
- antagonistes et inhibiteurs : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- pharmacologie : Protéines recombinantes.
- physiologie : Virus du SRAS.
- Dichroïsme circulaire, Fusion membranaire, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Électrophorèse sur gel de polyacrylamide.

English descriptors

KwdEn :
- Cell Line, Circular Dichroism, Electrophoresis, Polyacrylamide Gel, Humans, Membrane Fusion (drug effects), Membrane Glycoproteins (antagonists & inhibitors), Recombinant Proteins (pharmacology), SARS Virus (physiology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (antagonists & inhibitors).
MESH :
- chemical , antagonists & inhibitors : Membrane Glycoproteins, Viral Envelope Proteins.
- drug effects : Membrane Fusion.
- chemical , pharmacology : Recombinant Proteins.
- physiology : SARS Virus.
- Cell Line, Circular Dichroism, Electrophoresis, Polyacrylamide Gel, Humans, Spike Glycoprotein, Coronavirus.

Abstract

Entry of SARS-CoV into a target cell is initiated by binding of the S1 domain of spike protein to a receptor, followed by conformational changes of the spike protein S2 domain, resulting in the formation of a six-helix bundle by the heptad-repeat (HR1 and HR2) regions. Our previous studies have demonstrated that peptides derived from HR2 region could inhibit SARS-CoV entry. However, synthesis of these peptides is at high cost. In this study, we designed two recombinant proteins, one containing two HR1 and one HR2 peptides (denoted HR121), and the other consisting of two HR2 and one HR1 peptides (designated HR212). These two proteins could be easily purified with the low cost of production, exhibiting high stability and potent inhibitory activity on entry of the HIV/SARS pseudoviruses with IC(50) values of 4.13 and 0.95muM, respectively. These features suggest that HR121 and HR212 can serve as potent inhibitors of SARS-CoV entry.

DOI: 10.1016/j.bbrc.2005.02.117
PubMed: 15781229

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Design of recombinant protein-based SARS-CoV entry inhibitors targeting the heptad-repeat regions of the spike protein S2 domain.</title>
<author><name sortKey="Ni, Ling" sort="Ni, Ling" uniqKey="Ni L" first="Ling" last="Ni">Ling Ni</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhu, Jieqing" sort="Zhu, Jieqing" uniqKey="Zhu J" first="Jieqing" last="Zhu">Jieqing Zhu</name>
</author>
<author><name sortKey="Zhang, Junjie" sort="Zhang, Junjie" uniqKey="Zhang J" first="Junjie" last="Zhang">Junjie Zhang</name>
</author>
<author><name sortKey="Yan, Meng" sort="Yan, Meng" uniqKey="Yan M" first="Meng" last="Yan">Meng Yan</name>
</author>
<author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name>
</author>
<author><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
<idno type="RBID">pubmed:15781229</idno>
<idno type="pmid">15781229</idno>
<idno type="doi">10.1016/j.bbrc.2005.02.117</idno>
<idno type="wicri:Area/PubMed/Corpus">002831</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002831</idno>
<idno type="wicri:Area/PubMed/Curation">002831</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002831</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002763</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002763</idno>
<idno type="wicri:Area/Ncbi/Merge">000E50</idno>
<idno type="wicri:Area/Ncbi/Curation">000E50</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000E50</idno>
<idno type="wicri:doubleKey">0006-291X:2005:Ni L:design:of:recombinant</idno>
<idno type="wicri:Area/Main/Merge">004B83</idno>
<idno type="wicri:Area/Main/Curation">004874</idno>
<idno type="wicri:Area/Main/Exploration">004874</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Design of recombinant protein-based SARS-CoV entry inhibitors targeting the heptad-repeat regions of the spike protein S2 domain.</title>
<author><name sortKey="Ni, Ling" sort="Ni, Ling" uniqKey="Ni L" first="Ling" last="Ni">Ling Ni</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhu, Jieqing" sort="Zhu, Jieqing" uniqKey="Zhu J" first="Jieqing" last="Zhu">Jieqing Zhu</name>
</author>
<author><name sortKey="Zhang, Junjie" sort="Zhang, Junjie" uniqKey="Zhang J" first="Junjie" last="Zhang">Junjie Zhang</name>
</author>
<author><name sortKey="Yan, Meng" sort="Yan, Meng" uniqKey="Yan M" first="Meng" last="Yan">Meng Yan</name>
</author>
<author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name>
</author>
<author><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name>
</author>
</analytic>
<series><title level="j">Biochemical and biophysical research communications</title>
<idno type="ISSN">0006-291X</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Line</term>
<term>Circular Dichroism</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Humans</term>
<term>Membrane Fusion (drug effects)</term>
<term>Membrane Glycoproteins (antagonists & inhibitors)</term>
<term>Recombinant Proteins (pharmacology)</term>
<term>SARS Virus (physiology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (antagonists & inhibitors)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Dichroïsme circulaire</term>
<term>Fusion membranaire ()</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (antagonistes et inhibiteurs)</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Protéines de l'enveloppe virale (antagonistes et inhibiteurs)</term>
<term>Protéines recombinantes (pharmacologie)</term>
<term>Virus du SRAS (physiologie)</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Membrane Fusion</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cell Line</term>
<term>Circular Dichroism</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Humans</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Dichroïsme circulaire</term>
<term>Fusion membranaire</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Entry of SARS-CoV into a target cell is initiated by binding of the S1 domain of spike protein to a receptor, followed by conformational changes of the spike protein S2 domain, resulting in the formation of a six-helix bundle by the heptad-repeat (HR1 and HR2) regions. Our previous studies have demonstrated that peptides derived from HR2 region could inhibit SARS-CoV entry. However, synthesis of these peptides is at high cost. In this study, we designed two recombinant proteins, one containing two HR1 and one HR2 peptides (denoted HR121), and the other consisting of two HR2 and one HR1 peptides (designated HR212). These two proteins could be easily purified with the low cost of production, exhibiting high stability and potent inhibitory activity on entry of the HIV/SARS pseudoviruses with IC(50) values of 4.13 and 0.95muM, respectively. These features suggest that HR121 and HR212 can serve as potent inhibitors of SARS-CoV entry.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<settlement><li>Pékin</li>
</settlement>
</list>
<tree><noCountry><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name>
<name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name>
<name sortKey="Yan, Meng" sort="Yan, Meng" uniqKey="Yan M" first="Meng" last="Yan">Meng Yan</name>
<name sortKey="Zhang, Junjie" sort="Zhang, Junjie" uniqKey="Zhang J" first="Junjie" last="Zhang">Junjie Zhang</name>
<name sortKey="Zhu, Jieqing" sort="Zhu, Jieqing" uniqKey="Zhu J" first="Jieqing" last="Zhu">Jieqing Zhu</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Ni, Ling" sort="Ni, Ling" uniqKey="Ni L" first="Ling" last="Ni">Ling Ni</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004874 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004874 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:15781229
   |texte=   Design of recombinant protein-based SARS-CoV entry inhibitors targeting the heptad-repeat regions of the spike protein S2 domain.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:15781229" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

Serveur d'exploration SRAS

Design of recombinant protein-based SARS-CoV entry inhibitors targeting the heptad-repeat regions of the spike protein S2 domain.

Design of recombinant protein-based SARS-CoV entry inhibitors targeting the heptad-repeat regions of the spike protein S2 domain.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.